Lyophilization of Brucella melitensis

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Lyophilization of Brucella melitensis1

The preservation of Brucella melitensis was of initerest because Elberg and Faunce (1957) had shown that a nondependent mutant derived from a streptomycindepenident strain of B. melitensis was immunogeniic wheni administered as a living vaccine. Huttoni et al. (1951) and, more recenitly, Alexander and van Drimmelen (1956), showed that lyophilization was practical for preserving the viability of...

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Brucella peritonitis and leucocytoclastic vasculitis due to Brucella melitensis.

Brucellosis is a multisystemic disease that rarely leads to a fatal outcome. While reticuloendothelial system organs are mostly affected, peritonitis and posthepatitic cirrhosis are also complications of brucellosis, though they are very rare. Brucella spp. can also trigger immunological reactions. We report a case of brucellosis with peritonitis, renal failure and leucocytoclastic vasculitis c...

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Antigenic relationship of Brucella ovis and Brucella melitensis.

Immune sera were prepared in rabbits by the injection of living and acetone-killed cells of Brucella ovis and smooth and rough B. melitensis. The use of whole-cell antigens in agglutination and agglutinin-absorption tests revealed little relationship between B. ovis and smooth B. melitensis, although there was extensive cross-agglutination between B. ovis and rough B. melitensis. The use of wat...

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Oral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis 16M.

Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of conjunctiva or traumatized skin by infected animal products. In addition, Brucella is recognized as a biowarfare threat agent. Although a vaccine to protect humans from natural or deliberate infection could be useful, vaccines presently used in animals are unsuitable for human use. We te...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1960

ISSN: 0003-6919

DOI: 10.1128/am.8.1.52-54.1960